Molecular identification of Paramphistomidae obtained from ruminants in Van province

This study was aimed to identify Paramphistomum species from infected ruminants (sheep and cattle) by molecular methods between March 2018 September at Van municipality slaughterhouse. In the research, rDNA ITS-2 gene region of adult Paramphistomums amplified PCR method. Amplicons 399 bp long were viewed in agarose gel. As a result bidirectional sequence analysis made amplicons, leydeni Calicophoron daubneyi identifyed. Amplicon sequences compared BLAST with reference Genbank. Phylogenetic tree created Neighbor-Joining method using MEGA 7 program. Genotypes obtained isolates for exact or closest similarities. conclusion, C. daubneyi, which previously detected morphological Turkey, this study, it identified first time methods. Also P. reported Turkey.